Mimouna Yakoubi1,2, Nasser Belboukhari1, Khaled Sekkoum1, Hamid Benlakhdar1, Mohammed Bouchekara1, Hassan Y. Aboul-Enein3*
1Bioactive Molecules and Chiral Separation Laboratory, University of Bechar, PO Box 417, Bechar 08000, Algeria
2Process Engineering and Solution Chemistry Laboratory, University Mustapha Stambouli of Mascara, PO Box 305, Mascara 29000, Algeria
3Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Center, Dokki, Giza 12622, Egypt
*Corresponding author: Hassan Y. Aboul-Enein, Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Center, Dokki, Giza 12622, Egypt, E-mail: [email protected]
Received Date: August 09, 2021
Publication Date: September 09, 2021
Objective: A known sesquiterpenes β-Eudesmol (beta-Selinenol) the main components of essential oil of Warionia saharae have been isolated from the hydrodistillation extract leaves. The essential oil components was analyzed and identified by GC-MS spectroscopy.
Methodology: The main chiral component has been chromatographically purified by TLC and GLC by analysis of their spectroscopic data (GC-MS, IR) and by using vibrational circular dichroism (VCD) spectroscopy associated with density functional theory (DFT) calculations to confirm its structure and absolute configuration.
Results and discussion: VCD has attracted increasing attention in recent years; it is a definitive method, commonly used in the absolute configuration of natural products. While the most of major components of the essential oil of WS are optically active molecules such as β-eudesmol, we carried out in this work a comparison between the observed spectra of the essential oil and VCD spectra reported in the literature of each pure enantiomer.
Keywords: Warionia saharae; Essential oil; Isolation; β-Eudesmol; VCD analysis